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Methods Enzymol. 1983;101:228-45.

Genetic applications of yeast transformation with linear and gapped plasmids.

Abstract

Techniques for high frequency yeast transformation have been described. A double-strand break introduced by restriction enzyme cleavage can be used to direct a plasmid to integrate into a particular chromosomal locus. Plasmids containing a double-strand gap can be used in a straightforward method for the isolation and mapping of chromosomal alleles. These techniques extend the genetic applications of yeast transformation.

PMID:
6310326
[PubMed - indexed for MEDLINE]
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