Gene. 1982 Oct;19(3):327-36.

Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5.

Beck E, Ludwig G, Auerswald EA, Reiss B, Schaller H.

Abstract

The nucleotide sequence of 1200 bp from the unique region of transposon Tn5 containing the neomycin phosphotransferase gene (neo) was determined, and the location of the neo gene was identified by deletion mutants in a translational reading frame of 792 bp. The derived gene product, an aminoglycoside 3'-phosphotransferase (APH) II, consists of 264 amino acid residues and has a calculated Mr of 29053. Its amino acid sequence shows sequence homologies to the APH type I enzyme coded for by transposon Tn903 (Oka et al., 1981).

PMID:: 6295884; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Secondary Source ID

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

Secondary Source ID

GENBANK/J01834

LinkOut - more resources

Medical

Genes and Gene Therapy - MedlinePlus Health Information

Supplemental Content

Related citations

Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids. [Proc Natl Acad Sci U S A. 1983]
Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids.
Thompson CJ, Gray GS. Proc Natl Acad Sci U S A. 1983 Sep; 80(17):5190-4.
Nucleotide sequence of the Streptococcus faecalis plasmid gene encoding the 3'5"-aminoglycoside phosphotransferase type III. [Gene. 1983]
Nucleotide sequence of the Streptococcus faecalis plasmid gene encoding the 3'5"-aminoglycoside phosphotransferase type III.
Trieu-Cuot P, Courvalin P. Gene. 1983 Sep; 23(3):331-41.
Construction and uses of a new transposable element whose insertion is able to produce gene fusions with the neomycin-phosphotransferase-coding region of Tn903. [Gene. 1986]
Construction and uses of a new transposable element whose insertion is able to produce gene fusions with the neomycin-phosphotransferase-coding region of Tn903.
Ratet P, Richaud F. Gene. 1986; 42(2):185-92.
Review Developing a snapshot of the ATP binding domain(s) of aminoglycoside phosphotransferases. [Front Biosci. 1999]
Review Developing a snapshot of the ATP binding domain(s) of aminoglycoside phosphotransferases.
Perlin MH, Brown SA, Dholakia JN. Front Biosci. 1999 Jan 1; 4:D63-71. Epub 1999 Jan 1.
Review [Restriction enzymes. II. Mechanisms of action of type I and III restriction-modyfication systems (author's transl)]. [Postepy Biochem. 1980]
Review [Restriction enzymes. II. Mechanisms of action of type I and III restriction-modyfication systems (author's transl)].
Stasiak A. Postepy Biochem. 1980; 26(3):369-87.

See reviews... See all...

Cited by over 100 PubMed Central articles

Novel sulI binary vectors enable an inexpensive foliar selection method in Arabidopsis. [BMC Res Notes. 2011]
Novel sulI binary vectors enable an inexpensive foliar selection method in Arabidopsis.
Thomson JG, Cook M, Guttman M, Smith J, Thilmony R. BMC Res Notes. 2011 Mar 2; 4:44. Epub 2011 Mar 2.
Review Aminoglycoside modifying enzymes. [Drug Resist Updat. 2010]
Review Aminoglycoside modifying enzymes.
Ramirez MS, Tolmasky ME. Drug Resist Updat. 2010 Dec; 13(6):151-71. Epub 2010 Sep 15.
Isolation and characterization of a suppressor mutation that restores Myxococcus xanthus exopolysaccharide production. [Microbiology. 2009]
Isolation and characterization of a suppressor mutation that restores Myxococcus xanthus exopolysaccharide production.
Black WP, Xu Q, Cadieux CL, Suh SJ, Shi W, Yang Z. Microbiology. 2009 Nov; 155(Pt 11):3599-610. Epub 2009 Aug 14.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Nucleotide sequence and exact localization of the neomycin phosphotransferase ge...
Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5.
Gene. 1982 Oct ;19(3):327-36.

PubMed
Nucleotide sequence of the hygromycin B phosphotransferase gene from Streptomyce...
Nucleotide sequence of the hygromycin B phosphotransferase gene from Streptomyces hygroscopicus.
Nucleic Acids Res. 1986 Feb 25 ;14(4):1565-81.

PubMed
Isolation and characterization of efficient plasmid transformation mutants of My...
Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis.
Mol Microbiol. 1990 Nov ;4(11):1911-9.

PubMed
New and versatile cloning vectors with kanamycin-resistance marker.
New and versatile cloning vectors with kanamycin-resistance marker.
Gene. 1987 ;56(2-3):309-12.

PubMed
Prostate cancer risk and prediagnostic serum 25-hydroxyvitamin D levels (Finland...
Prostate cancer risk and prediagnostic serum 25-hydroxyvitamin D levels (Finland).
Cancer Causes Control. 2000 Oct ;11(9):847-52.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to: