Physical and genetic organization of the IncN-group plasmid pCU1

Gene. 1981 Aug;14(3):195-204. doi: 10.1016/0378-1119(81)90115-3.

Abstract

A restriction endonuclease-cleavage map of the IncN group plasmid pCU1 was constructed. Deletion mutants of the plasmid were obtained by in vivo or in vitro methods. Comparison of the restriction maps of these mutants to that of pCU1 enables one to assign the known functions of the plasmid to particular regions on the plasmid DNA. For different enzymes, the number and distribution of restriction sites on pCU1 is compared to that of other IncN and related plasmids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Restriction Enzymes / metabolism
  • DNA, Viral / genetics*
  • Electrophoresis, Polyacrylamide Gel
  • Mutation*
  • Phenotype
  • Plasmids*

Substances

  • DNA, Viral
  • DNA Restriction Enzymes