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Long term culture of bovine oligodendroglia isolated with a Percoll gradient.
Oligodendroglia were isolated from calf central nervous system (CNS) white matter by trypsinization in phosphate buffered saline and separation by centrifugation through Percoll. Using antisera to phenotypic markers and double labelling experiments we were able to identify essentially all cells in the cultures. The cells obtained were: (1) viable; (2) had intact plasma membranes and well preserved organelles, ribosomes and mitochondria; and (3) were greater than or equal to 95% oligodendroglia 16-20 h after isolation as determined by ability to bind antigalactocerebroside antibodies (anti-GalC). Oligodendroglia could be cultured for several weeks to months. Oligodendroglia established and maintained processes which bound anti-GalC. Myelin basic protein could be demonstrated in the cytoplasm of 40-60% of oligodendroglia cell bodies but not in the processes.
PMID: 6269698 [PubMed - indexed for MEDLINE]
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Cited by 3 PubMed Central articles
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Lipid metabolism of isolated oligodendrocytes maintained in long-term culture mimics events associated with myelinogenesis.
Szuchet S, Yim SH, Monsma S.
Proc Natl Acad Sci U S A. 1983 Nov; 80(22):7019-23.
[Proc Natl Acad Sci U S A. 1983]
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Neurosteroids: oligodendrocyte mitochondria convert cholesterol to pregnenolone.
Hu ZY, Bourreau E, Jung-Testas I, Robel P, Baulieu EE.
Proc Natl Acad Sci U S A. 1987 Dec; 84(23):8215-9.
[Proc Natl Acad Sci U S A. 1987]
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The M2 autoantigen of central nervous system myelin, a glycoprotein present in oligodendrocyte membrane.
Lebar R, Lubetzki C, Vincent C, Lombrail P, Boutry JM.
Clin Exp Immunol. 1986 Nov; 66(2):423-34.
[Clin Exp Immunol. 1986]