Expression of a bacterial gene in mammalian cells

Science. 1980 Sep 19;209(4463):1422-7. doi: 10.1126/science.6251549.

Abstract

Transfection of cultured monkey kidney cells with recombinant DNA constructed with a cloned Escherichia coli gene that codes for xanthine-guanine phosphoribosyltransferase and several different SV40 DNA-based vectors, results in the synthesis of readily measurable quantities of the bacterial enzyme. Moreover, the physiological defect in purine nucleotide synthesis characteristic of human Lesch-Nyhan cells can be overcome by the introduction of the bacterial gene into these cells.

MeSH terms

  • Animals
  • Cells, Cultured
  • Cloning, Molecular / methods
  • DNA, Bacterial / genetics*
  • DNA, Recombinant*
  • Escherichia coli
  • Genes*
  • Haplorhini
  • Humans
  • Hypoxanthine Phosphoribosyltransferase / genetics
  • Lesch-Nyhan Syndrome / genetics*
  • Pentosyltransferases / genetics*
  • Simian virus 40 / genetics
  • Transduction, Genetic
  • Transformation, Genetic

Substances

  • DNA, Bacterial
  • DNA, Recombinant
  • Pentosyltransferases
  • xanthine phosphoribosyltransferase
  • Hypoxanthine Phosphoribosyltransferase