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Biochem J. 1980 Jan 1;185(1):59-63.

The isolation and characterization of a sequence-specific endonuclease from Anabaena subcylindrica.

Abstract

An endonuclease, AsuI, was isolated from extracts of Anabaena subcylindrica on the basis of gel-electrophoretic analysis of digests of bacteriophage-lambda DNA with the paritally purified extracts. The enzyme requires Mg2+, but no other cofactors. Endonuclease AsuI recognizes the interrupted tetranucleotide sequence: (Formula: see tex), and breaks the phosphodiester bonds indicated by the arrows to leave single-stranded trinucleotide projections at the 5'-termini of the DNA fragments.

PMID:
6246880
PMCID:
PMC1161269
[PubMed - indexed for MEDLINE]
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