Docosahexaenoic acid (22:6, n-3) is metabolized to lipoxygenase reaction products in the retina

Biochem Biophys Res Commun. 1984 Dec 14;125(2):741-7. doi: 10.1016/0006-291x(84)90601-6.

Abstract

Docosahexaenoic acid (22:6, n-3), a major component of retinal phospholipids, is a substrate for active lipoxygenation in intact canine retinas incubated in vitro with [U-14C]docosahexaenoic acid. The major lipoxygenase reaction product was identified by high performance liquid chromatography and gas chromatography-mass spectrometry as 11-hydroxy-4,7,9-(trans)13,16,19 docosahexaenoic acid. Other mono- and di-hydroxy derivatives also were detected. The synthesis of these compounds was inhibited by the antioxidant and lipoxygenase inhibitor, nordihydroguaiaretic acid, but was not inhibited by indomethacin or esculetin.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Carbon Radioisotopes
  • Catechols / pharmacology
  • Chromatography, High Pressure Liquid
  • Docosahexaenoic Acids
  • Dogs
  • Fatty Acids, Unsaturated / metabolism*
  • Indomethacin / pharmacology
  • Lipoxygenase / metabolism*
  • Masoprocol
  • Mass Spectrometry
  • Retina / drug effects
  • Retina / metabolism*
  • Umbelliferones / pharmacology

Substances

  • Carbon Radioisotopes
  • Catechols
  • Fatty Acids, Unsaturated
  • Umbelliferones
  • Docosahexaenoic Acids
  • Masoprocol
  • Lipoxygenase
  • esculetin
  • Indomethacin