Subsets of blood, spleen and recirculating lymphocytes in man

Clin Exp Immunol. 1983 Sep;53(3):672-8.

Abstract

Lymphocyte subpopulations were characterized in human blood and spleens. In addition the spleens were perfused by a closed extracorporeal perfusion system under almost physiological conditions. Lymphocytes released from the spleen during perfusion were taken to be representative of recirculating lymphocytes. B lymphocytes were classified by their surface immunoglobulin, T lymphocytes and T lymphocyte subsets by cytochemistry, sheep red blood cell rosette formation and in some experiments by monoclonal antibodies. In the blood 71 +/- 4.3% of the lymphocytes were rosette forming cells and 23.3 +/- 3.8% B lymphocytes. In the spleen 49.8 +/- 3.6% were T and 53.3 +/- 2.1% were B lymphocytes. In three spleens the mean number of OKT3+ lymphocytes were 27.6 +/- 7.0% OKT4+ 8.6 +/- 1.4% and OKT8+ 13.7 +/- 2.2%. The ratio of T helper to T suppressor lymphocytes was 0.67 for the spleen and 1.7 for the blood. The lymphocytes released from the perfused spleen showed a similar distribution pattern of surface markers to that of the splenic subpopulations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Antibodies, Monoclonal / immunology
  • B-Lymphocytes
  • Humans
  • Leukocyte Count
  • Lymphocytes / classification*
  • Middle Aged
  • Naphthol AS D Esterase / analysis
  • Perfusion
  • Receptors, Antigen, B-Cell / analysis
  • Rosette Formation
  • Spleen / cytology*
  • T-Lymphocytes / classification
  • T-Lymphocytes, Helper-Inducer
  • T-Lymphocytes, Regulatory

Substances

  • Antibodies, Monoclonal
  • Receptors, Antigen, B-Cell
  • Naphthol AS D Esterase