Vibrational effects have been found to be associated with increased frequency of low-back pain in various industries, and because of this the study herein reported was conducted to assess the physiologic response of the spinal system to sinusoidal vibrations. A device replicating industrial vibration was constructed and employed on healthy human volunteers to determine the stiffness, impedence, and resonant characteristics of the subjects. Three peaks of enhanced transmissibility corresponding to the resonant frequency of the spinal system were found. The greatest transmissibility of vibratory input occurs at the first resonant frequency (5Hz) where marked enhancement of vibrational input occurs as vibrations pass through the spinal system. A progressive stiffening of the system occurs in response to vibrational inputs of increasing frequency. The effects of posture, the Valsalva maneuver, and fatigue alter the normal response. Structures vibrated at the first resonant frequency have greater potential for damage.