Science. 1984 Nov 23;226(4677):972-4.

Detection of c-sis transcripts and synthesis of PDGF-like proteins by human osteosarcoma cells.

Graves DT, Owen AJ, Barth RK, Tempst P, Winoto A, Fors L, Hood LE, Antoniades HN.

Abstract

Platelet-derived growth factor (PDGF) has been previously shown to be homologous to the transforming gene of simian sarcoma virus (v-sis), and inappropriate expression of the cellular counterpart of the v-sis gene (c-sis) has been implicated in the generation of mesenchymal tumors. The U-2 OS human osteosarcoma line was shown to contain multiple c-sis transcripts. Immunoprecipitation experiments with antiserum to PDGF identified a variety of polypeptides ranging in size from 18,000 to 165,000 daltons that were immunoprecipitated specifically from U-2 OS cell extracts. The osteosarcoma also was shown to secrete a 29,000-dalton protein having the serological and structural characteristics of PDGF.

PMID:: 6209798; [PubMed - indexed for MEDLINE]

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Detection of c-sis transcripts and synthesis of PDGF-like proteins by human osteosarcoma cells.

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