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A method for isolation of intact, translationally active ribonucleic acid.
A method for isolation of large, translationally active RNA species is presented. The procedure involves homogenization of cells or tissues in 5 M guanidine monothiocyanate followed by direct precipitation of RNA from the guanidinium by 4 M LiCl. Modifications are described for use with tissue culture cells, yeast, tissues, or isolated nuclei. The advantages of the procedure include speed, simplicity, avoidance of an ultracentrifugation, and its applicability to large numbers of small samples. The procedure yields large mRNA precursors up to 10 kb and mRNA species which translate very well. However, small (less than 300 nucleotides) RNA species are recovered with a poor yield.
PMID: 6198133 [PubMed - indexed for MEDLINE]
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Cited by over 100 PubMed Central articles
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TRBP control of PACT-induced phosphorylation of protein kinase R is reversed by stress.
Daher A, Laraki G, Singh M, Melendez-Peña CE, Bannwarth S, Peters AH, Meurs EF, Braun RE, Patel RC, Gatignol A.
Mol Cell Biol. 2009 Jan; 29(1):254-65. Epub 2008 Oct 20.
[Mol Cell Biol. 2009]
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Genetic screens for mutations affecting development of Xenopus tropicalis.
Goda T, Abu-Daya A, Carruthers S, Clark MD, Stemple DL, Zimmerman LB.
PLoS Genet. 2006 Jun; 2(6):e91. Epub 2006 Jun 9.
[PLoS Genet. 2006]
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Active Translation of the D-1 Protein of Photosystem II in Senescing Leaves.
Droillard MJ, Bate NJ, Rothstein SJ, Thompson JE.
Plant Physiol. 1992 Jun; 99(2):589-594.
[Plant Physiol. 1992]
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Patient Drug Information
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Lithium (Eskalith CR® , Eskalith® , Lithobid® )
Lithium is used to treat and prevent episodes of mania (frenzied, abnormally excited mood) in people with bipolar disorder (manic depressive disorder; a disease that causes episodes of depression, episodes of mania, and ...