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High-resolution chromosomal localization of human genes for amylase, proopiomelanocortin, somatostatin, and a DNA fragment (D3S1) by in situ hybridization.
The method of in situ hybridization has become a significant technique for specific-site chromosome mapping. We show that the resolution of in situ hybridization can be increased by hybridizing the probe to stretched prometaphase chromosomes with high-resolution banding obtained after 5-bromodeoxyuridine treatment of the cells and with a Hoechst 33258/Giemsa chromosome-staining method. Using this procedure, we assigned to specific chromosome sites three cloned genes and one DNA polymorphism: amylase gene (AMY) to 1p21; proopiomelanocortin gene (POMC) to 2p23, somatostatin gene (SST) to 3q28, and a single copy DNA segment (D3S1) to 3q12.
PMID: 6196780 [PubMed - indexed for MEDLINE]
PMCID: PMC390100
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Cited by 26 PubMed Central articles
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Targeted gene walking by low stringency polymerase chain reaction: assignment of a putative human brain sodium channel gene (SCN3A) to chromosome 2q24-31.
Malo MS, Srivastava K, Andresen JM, Chen XN, Korenberg JR, Ingram VM.
Proc Natl Acad Sci U S A. 1994 Apr 12; 91(8):2975-9.
[Proc Natl Acad Sci U S A. 1994]
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Chromosome deletion mapping of interspersed low-copy repetitive DNA.
Funderburk SJ, Sparkes RS, Klisak I, Law ML.
Am J Hum Genet. 1984 Jul; 36(4):769-76.
[Am J Hum Genet. 1984]
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Chromosomal locations of the human and mouse genes for precursors of epidermal growth factor and the beta subunit of nerve growth factor.
Zabel BU, Eddy RL, Lalley PA, Scott J, Bell GI, Shows TB.
Proc Natl Acad Sci U S A. 1985 Jan; 82(2):469-73.
[Proc Natl Acad Sci U S A. 1985]
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