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Bacterial synthesis of a novel human leukocyte interferon.
A novel human leukocyte interferon cDNA clone (LeIF B) was identified in a cDNA library prepared using polyadenylated mRNA of a myeloblastoid cell line. The nucleotide sequence of LeIF B differs significantly from other published leukocyte interferon cDNA sequences. An expression plasmid was constructed which directs the synthesis in E. coli of 8 x 10(7) interferon units per liter of culture. LeIF B exhibits markedly different specificities from another bacterially synthesized human leukocyte interferon, LeIF A.
PMID: 6164048 [PubMed - indexed for MEDLINE]
PMCID: PMC327233
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Cited by 12 PubMed Central articles
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High-level expression of an interferon alpha 2 gene cloned in phage M13mp7 and subsequent purification with a monoclonal antibody.
Slocombe P, Easton A, Boseley P, Burke DC.
Proc Natl Acad Sci U S A. 1982 Sep; 79(18):5455-9.
[Proc Natl Acad Sci U S A. 1982]
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Expression of human interferon genes using the recA promoter of Escherichia coli.
Feinstein SI, Chernajovsky Y, Chen L, Maroteaux L, Mory Y.
Nucleic Acids Res. 1983 May 11; 11(9):2927-41.
[Nucleic Acids Res. 1983]
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High-level expression of a chemically synthesized gene for human interferon-gamma using a prokaryotic expression vector.
Jay E, Rommens J, Pomeroy-Cloney L, MacKnight D, Lutze-Wallace C, Wishart P, Harrison D, Lui WY, Asundi V, Dawood M.
Proc Natl Acad Sci U S A. 1984 Apr; 81(8):2290-4.
[Proc Natl Acad Sci U S A. 1984]
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