Nucleic Acids Res. 1980 Jul 11;8(13):2885-94.

The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide primers of reverse transcriptase.

Houghton M, Easton MA, Stewart AG, Smith JC, Doel SM, Catlin GH, Lewis HM, Patel TP, Emtage JS, Carey NH, Porter AG.

Abstract

Using synthetic oligodeoxyribonucleotides to prime the transcription of interferon mRNA and cDNA, we recently determined the mRNA sequence coding for the 47 amino-terminal amino acids of mature human fibroblast interferon (1). From this sequence, we have now synthesised an oligodeoxyribonucleotide that is homologous with the mRNA sequence coding for amino acids 42-45 and used it as a primer to selectively transcribe an interferon cDNA template. The sequence of the newly synthesised DNA predicted the sequence of amino acids 48-109 in the interferon polypeptide. By repeating this process with one more primer, we have determined the complete amino acid sequence of mature human fibroblast interferon, a polypeptide of 166 amino acids.

PMID:: 6159580; [PubMed - indexed for MEDLINE]
PMCID:: PMC324132

Free PMC Article

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

The amino-terminal sequence of human fibroblast interferon as deduced from reverse transcripts obtained using synthetic oligonucleotide primers. [Nucleic Acids Res. 1980]
The amino-terminal sequence of human fibroblast interferon as deduced from reverse transcripts obtained using synthetic oligonucleotide primers.
Houghton M, Stewart AG, Doel SM, Emtage JS, Eaton MA, Smith JC, Patel TP, Lewis HM, Porter AG, Birch JR, et al. Nucleic Acids Res. 1980 May 10; 8(9):1913-31.
Synthesis of human fibroblast interferon by E. coli. [Nucleic Acids Res. 1980]
Synthesis of human fibroblast interferon by E. coli.
Goeddel DV, Shepard HM, Yelverton E, Leung D, Crea R, Sloma A, Pestka S. Nucleic Acids Res. 1980 Sep 25; 8(18):4057-74.
Use of oligodeoxynucleotide primers to determine poly(adenylic acid) adjacent sequences in messenger ribonucleic acid. 3'-Terminal noncoding sequence of bovine growth hormone messenger ribonucleic acid. [Biochemistry. 1980]
Use of oligodeoxynucleotide primers to determine poly(adenylic acid) adjacent sequences in messenger ribonucleic acid. 3'-Terminal noncoding sequence of bovine growth hormone messenger ribonucleic acid.
Sasavage NL, Smith M, Gillam S, Astell C, Nilson JH, Rottman F. Biochemistry. 1980 Apr 29; 19(9):1737-43.
Review Isolation and characterization of a human fibroblast interferon gene and its expression in Escherichia coli. [Rev Infect Dis. 1981]
Review Isolation and characterization of a human fibroblast interferon gene and its expression in Escherichia coli.
Derynck R, Devos R, Remaut E, Saman E, Stanssens P, Tavernier J, Volckaert G, Content J, De Clercq E, Fiers W. Rev Infect Dis. 1981 Nov-Dec; 3(6):1186-95.
Review [Cytogenetics of the human interferon system]. [Zh Mikrobiol Epidemiol Immunob...]
Review [Cytogenetics of the human interferon system].
Khesin IaE. Zh Mikrobiol Epidemiol Immunobiol. 1984 Oct; (10):6-12.

See reviews... See all...

Cited by 9 PubMed Central articles

Regulated expression of human interferon beta 1 gene after transduction into cultured mouse and rabbit cells. [Proc Natl Acad Sci U S A. 1982]
Regulated expression of human interferon beta 1 gene after transduction into cultured mouse and rabbit cells.
Canaani D, Berg P. Proc Natl Acad Sci U S A. 1982 Sep; 79(17):5166-70.
Human fibroblast interferon RNA transcripts of different sizes in poly(I).poly(C) induced cells. [Nucleic Acids Res. 1983]
Human fibroblast interferon RNA transcripts of different sizes in poly(I).poly(C) induced cells.
Content J, De Wit L, Tavernier J, Fiers W. Nucleic Acids Res. 1983 May 11; 11(9):2627-38.
Computer selection of oligonucleotide probes from amino acid sequences for use in gene library screening. [Nucleic Acids Res. 1984]
Computer selection of oligonucleotide probes from amino acid sequences for use in gene library screening.
Yang JH, Ye JH, Wallace DC. Nucleic Acids Res. 1984 Jan 11; 12(1 Pt 2):837-43.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Gene (OMIM)
Gene records associated with Online Mendelian Inheritance in Man (OMIM) records that cite the current articles in their reference lists.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
OMIM (calculated)
Online Mendelian Inheritance in Man (OMIM) records that include the current articles as references in the light bulb links within or in the citations at the end of the OMIM record. The references available through the light bulb link are collected using the PubMed related articles algorithm to identify records with similar terminology to the OMIM record.
OMIM (cited)
Online Mendelian Inheritance in Man (OMIM) records that include the current articles as reference cited at the end of the OMIM record.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

The complete amino acid sequence of human fibroblast interferon as deduced using...
The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide primers of reverse transcriptase.
Nucleic Acids Res. 1980 Jul 11 ;8(13):2885-94.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide primers of reverse transcriptase.

Abstract

MeSH Terms, Substances, Secondary Source ID

MeSH Terms

Substances

Secondary Source ID

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Cited by 9 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information