ATP-citrate lyase and other enzymes of acetyl-CoA metabolism in fractions of small and large synaptosomes from rat brain hippocampus and cerebellum

J Neurochem. 1983 Nov;41(5):1502-5. doi: 10.1111/j.1471-4159.1983.tb00854.x.

Abstract

The activities of choline acetyltransferase and ATP-citrate lyase were significantly correlated (r = 0.995) in fractions of small and large synaptosomes isolated from rat hippocampus and cerebellum. The activities of these two enzymes did not correlate with those of pyruvate dehydrogenase, carnitine acetyltransferase, citrate synthase, acetyl-CoA synthetase, lactate dehydrogenase, or with the rate of high-affinity glutamate uptake in the synaptosomal fractions. The results provide additional evidence linking ATP-citrate lyase to the cholinergic system in the brain.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ATP Citrate (pro-S)-Lyase / metabolism*
  • Acetyl Coenzyme A / metabolism*
  • Animals
  • Brain / enzymology*
  • Cerebellum / enzymology
  • Choline O-Acetyltransferase / metabolism
  • Glutamates / metabolism
  • Glutamic Acid
  • Hippocampus / enzymology
  • L-Lactate Dehydrogenase / metabolism
  • Male
  • Rats
  • Rats, Inbred Strains
  • Synaptosomes / enzymology*

Substances

  • Glutamates
  • Glutamic Acid
  • Acetyl Coenzyme A
  • L-Lactate Dehydrogenase
  • Choline O-Acetyltransferase
  • ATP Citrate (pro-S)-Lyase