[Fractionation of chromatin of liver cell nuclei after mild micrococcal nuclease digestion]

Mol Biol (Mosk). 1982 Mar-Apr;16(2):392-7.
[Article in Russian]

Abstract

Mild micrococcal nuclease treatment of rat and mouse nuclei and fractionation were based on the method of Tata and Baker. Three chromatin fractions, S, P1, P2, were separated, and for each of these fractions the sensitivity to the DNase 1 action was determined. The relative content in these fractions of non-transcribed DNA sequences was established by hydridization with a mouse satellite DNA, and the relative content of transcribed DNA sequences--by hydridization with DNA synthesised on the total poly (A) mRNA. None of the fractions displayed the properties characteristic of active chromatin.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Base Sequence
  • Cell Nucleus / analysis
  • Chromatin / isolation & purification*
  • Chromatin / metabolism
  • DNA / genetics*
  • DNA, Satellite / genetics
  • Kinetics
  • Liver / metabolism*
  • Mice
  • Micrococcal Nuclease
  • Nucleic Acid Hybridization
  • Poly A / genetics
  • RNA, Messenger / genetics
  • Rats
  • Transcription, Genetic

Substances

  • Chromatin
  • DNA, Satellite
  • RNA, Messenger
  • Poly A
  • DNA
  • Micrococcal Nuclease