-
The microbial oxidation of methanol. The prosthetic group of the alcohol dehydrogenase of Pseudomonas sp. M27: a new oxidoreductase prosthetic group.
1. The purified alcohol dehydrogenase of Pseudomonas sp. M27, whose action is independent of nicotinamide nucleotides, has absorption peaks at 280mmu and at 350mmu with little or no absorption at or above 450mmu. 2. It does not fluoresce, but green-fluorescent material, diffusible on dialysis, is produced when the enzyme is treated with acid or alkali or when it is boiled. 3. Evidence is presented that the enzyme is not a flavoprotein. 4. Kinetic studies show a correlation between enzyme inactivation by acid, alkali or heat and liberation of the fluorescent material. 5. Some purification of the fluorescent material was achieved, but definite identification was not possible; the major component has a fluorescence maximum at about 460mmu with excitation maxima at about 260mmu and 365mmu. 6. Data are given (including absorption and fluorescence spectra) that support the suggestion that the prosthetic group of the enzyme is a pteridine derivative. 7. Possible mechanisms of action of the enzyme are discussed.
PMID: 6049934 [PubMed - indexed for MEDLINE]
PMCID: PMC1271238
-
Cited by 30 PubMed Central articles
-
Characterization of a novel methanol dehydrogenase in representatives of Burkholderiales: implications for environmental detection of methylotrophy and evidence for convergent evolution.
Kalyuzhnaya MG, Hristova KR, Lidstrom ME, Chistoserdova L.
J Bacteriol. 2008 Jun; 190(11):3817-23. Epub 2008 Apr 4.
[J Bacteriol. 2008]
-
Substrate binding in quinoprotein ethanol dehydrogenase from Pseudomonas aeruginosa studied by electron-nuclear double resonance.
Kay CW, Mennenga B, Görisch H, Bittl R.
Proc Natl Acad Sci U S A. 2006 Apr 4; 103(14):5267-72. Epub 2006 Mar 27.
[Proc Natl Acad Sci U S A. 2006]
-
Catalytic mechanism of quinoprotein methanol dehydrogenase: A theoretical and x-ray crystallographic investigation.
Zheng YJ, Xia Zx, Chen Zw, Mathews FS, Bruice TC.
Proc Natl Acad Sci U S A. 2001 Jan 16; 98(2):432-4. Epub 2001 Jan 9.
[Proc Natl Acad Sci U S A. 2001]
- » See all...