Clin Chim Acta. 1979 Jul 16;95(2):189-200.

Determination of 3-mercaptolactate, mercaptoacetate and N-acetylcysteine in urine by gas chromatography.

Abstract

A method is described for the determination of 3-mercaptolactate, mercaptoacetate (thioglycolate) and N-acetylcysteine in urine. As these compounds are mainly excreted as their mixed disulfides with cysteine, they are first reduced to the free thiols by an insoluble polymer containing thiol groups. After purification by chromatography on an organomercurial adsorbent, the compounds are converted to benzyl derivatives by extractive alkylation and determined by gas chromatography. The identity of the compounds analyzed was verified by mass spectrometry. It was demonstrated that mercaptolactate and mercaptoacetate are almost entirely excreted as their mixed disulfides with cysteine, whereas appreciable amounts of N-acetylcyteine are present as the symmetrical disulfide and the free thiol. The urinary excretion of the compounds from healthy human beings was also studied.

PMID:: 527218; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Molecular Biology Databases

ACETYLCYSTEINE - HSDB

Supplemental Content

Save items

Related citations in PubMed

Reversed-phase ion-pair high-performance liquid chromatography of mercaptoacetate and N-acetylcysteine after derivatization with N-(1-pyrene)maleimide and N-(7-dimethylamino-4-methyl-3-coumarinyl)maleimide. [J Chromatogr. 1982]
Reversed-phase ion-pair high-performance liquid chromatography of mercaptoacetate and N-acetylcysteine after derivatization with N-(1-pyrene)maleimide and N-(7-dimethylamino-4-methyl-3-coumarinyl)maleimide.
Kågedal B, Källberg M. J Chromatogr. 1982 May 14; 229(2):409-15.
3-mercaptolactate cysteine disulfiduria: biochemical studies on affected and unaffected members of a family. [Biochem Med. 1981]
3-mercaptolactate cysteine disulfiduria: biochemical studies on affected and unaffected members of a family.
Hannestad U, Mårtensson J, Sjödahl R, Sörbo B. Biochem Med. 1981 Aug; 26(1):106-14.
Beta mercaptolactate-cysteine disulfide: analog of cystine in the urine of a mentally retarded patient. [Science. 1968]
Beta mercaptolactate-cysteine disulfide: analog of cystine in the urine of a mentally retarded patient.
Crawhall JC, Parker R, Sneddon W, Young EP, Ampola MG, Efron ML, Bixby EM. Science. 1968 Apr 26; 160(3826):419-20.
Determination of reduced, protein-unbound, and total concentrations of N-acetyl-L-cysteine and L-cysteine in rat plasma by postcolumn ligand substitution high-performance liquid chromatography. [Anal Biochem. 2001]
Determination of reduced, protein-unbound, and total concentrations of N-acetyl-L-cysteine and L-cysteine in rat plasma by postcolumn ligand substitution high-performance liquid chromatography.
Harada D, Naito S, Kawauchi Y, Ishikawa K, Koshitani O, Hiraoka I, Otagiri M. Anal Biochem. 2001 Mar; 290(2):251-9.
Review Determination of endogenous thiols and thiol drugs in urine by HPLC with ultraviolet detection. [J Chromatogr B Analyt Technol ...]
Review Determination of endogenous thiols and thiol drugs in urine by HPLC with ultraviolet detection.
Kuśmierek K, Chwatko G, Głowacki R, Bald E. J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Oct 15; 877(28):3300-8. Epub 2009 Mar 31.

See reviews... See all...

Cited by 1 PubMed Central article

N-acetylcysteine as an antidote in methylmercury poisoning. [Environ Health Perspect. 1998]
N-acetylcysteine as an antidote in methylmercury poisoning.
Ballatori N, Lieberman MW, Wang W. Environ Health Perspect. 1998 May; 106(5):267-71.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound
PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
OMIM (calculated)
Online Mendelian Inheritance in Man (OMIM) records that include the current articles as references in the light bulb links within or in the citations at the end of the OMIM record. The references available through the light bulb link are collected using the PubMed related articles algorithm to identify records with similar terminology to the OMIM record.
Substance
PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Determination of 3-mercaptolactate, mercaptoacetate and N-acetylcysteine in urin...
Determination of 3-mercaptolactate, mercaptoacetate and N-acetylcysteine in urine by gas chromatography.
Clin Chim Acta. 1979 Jul 16 ;95(2):189-200.

PubMed
Globoid cell leucodystrophy (Krabbe's disease): deficiency of galactocerebroside...
Globoid cell leucodystrophy (Krabbe's disease): deficiency of galactocerebroside beta-galactosidase.
Proc Natl Acad Sci U S A. 1970 Jun ;66(2):302-9.

PubMed
Anorexia nervosa. A psychiatric investigation of 94 female patients.
Anorexia nervosa. A psychiatric investigation of 94 female patients.
Acta Psychiatr Scand Suppl. 1970 ;214:1-194.

PubMed
Antigens of the Kell blood group system on neutrophils and monocytes: their rela...
Antigens of the Kell blood group system on neutrophils and monocytes: their relation to chronic granulomatous disease.
J Pediatr. 1975 Dec ;87(6 Pt 2):1117-20.

PubMed
Recovery of specific "caries-inducing" streptococci from carious lesions in the ...
Recovery of specific "caries-inducing" streptococci from carious lesions in the teeth of children.
Arch Oral Biol. 1970 May ;15(5):461-3.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: