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J Cell Biol. 1975 Sep;66(3):676-80.

Mouse-human heterokaryon analysis with a 33258 Hoechst-Giemsa technique.

Abstract

The bibenzimidazol derivative 33258 Hoechst can be used to distinguish microfluorometrically between mouse and human nuclei in heterokaryons. This affords a quick and accurate alternative to autoradiography in the analysis of such heterokaryons. The 33258 Hoechst fluorescence patterns can be converted after irradiation to a Giemsa rendition of the differential staining.

PMID:
51024
PMCID:
PMC2109466
[PubMed - indexed for MEDLINE]
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