Quantitative analysis of cholesterol in 5 to 20 microliter of plasma

J Lipid Res. 1974 May;15(3):286-91.

Abstract

A gas-liquid chromatographic micromethod for quantitation of cholesterol in 20 micro l of plasma was developed using 5alpha-cholestane as an internal standard, saponification with tetramethylammonium hydroxide-isopropanol, and extraction with tetrachloroethylene-methyl butyrate. Cholesterol levels in plasma samples were calculated by comparing cholesterol-cholestane peak height ratios with those of preassayed reference plasma. Over a plasma cholesterol range of 44 to 468 mg/100 ml, the gas-liquid chromatographic micromethod and the automated ferric chloride colorimetric method gave nearly identical results (r = 0.99) in duplicate aliquots of 131 plasma samples.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Analysis of Variance
  • Autoanalysis
  • Blood
  • Child
  • Cholesterol / blood*
  • Chromatography, Gas
  • Colorimetry
  • Evaluation Studies as Topic
  • Female
  • Humans
  • Indicators and Reagents
  • Mathematics
  • Methods
  • Microchemistry
  • Pregnancy
  • Time Factors
  • Umbilical Cord

Substances

  • Indicators and Reagents
  • Cholesterol