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1: Biochem J. 1973 Feb;131(2):199-209.Click here to read Links

Palmitoyl-coenzyme A synthetase. Mechanism of reaction.

Bar-Tana J, Rose G, Brandes R, Shapiro B.

The mechanism of long-chain fatty acid activation catalysed by highly purified microsomal palmitoyl-CoA synthetase was investigated. The kinetics of the overall reaction were found to conform to the Bi Uni Uni Bi Ping Pong mechanism. (18)O was transferred from [(18)O]palmitate to AMP and palmitoyl-CoA exclusively. The enzyme intermediate formed appeared to consist of enzyme-bound palmitate; this formation occurred only in the presence of ATP. However, the involvement of palmitoyl-AMP in the reaction catalysed by the purified enzyme has proved difficult to establish.

PMID: 4722436 [PubMed - indexed for MEDLINE]

PMCID: PMC1177459