J Lipid Res. 1972 Nov;13(6):824-6.

A simple lipase assay using trichloroacetic acid.

Abstract

An extremely rapid and sensitive assay for lipoprotein lipase activity, suitable for routine determinations, is described. The substrate for the assay is emulsified [2-(3)H]glyceryl trioleate, activated by serum. The method is based on trichloroacetic acid precipitation of unreacted substrate and measurement of (3)H-labeled glycerol.

PMID:: 4641423; [PubMed - indexed for MEDLINE]

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