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Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4.

Construction of biologically functional bacterial plasmids in vitro.

Abstract

The construction of new plasmid DNA species by in vitro joining of restriction endonuclease-generated fragments of separate plasmids is described. Newly constructed plasmids that are inserted into Escherichia coli by transformation are shown to be biologically functional replicons that possess genetic properties and nucleotide base sequences from both of the parent DNA molecules. Functional plasmids can be obtained by reassociation of endonuclease-generated fragments of larger replicons, as well as by joining of plasmid DNA molecules of entirely different origins.

PMID:
4594039
[PubMed - indexed for MEDLINE]
PMCID:
PMC427208
Free PMC Article
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