Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. IV. Characterization of the simian virus 40 ribonucleic acid species induced by wild-type simian virus 40 and by the nondefective hybrid virus, Ad2 + ND 1

J Virol. 1971 Aug;8(2):215-24. doi: 10.1128/JVI.8.2.215-224.1971.

Abstract

Ad2(+)ND(1), a nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid virus, has been previously shown to contain a small segment of the SV40 genome covalently linked to Ad2 deoxyribonucleic acid (DNA). The SV40 portion of this hybrid virus has been characterized by relating the SV40-specific ribonucleic acid (RNA) sequences transcribed from the Ad2(+)ND(1) DNA to those transcribed from the DNA of SV40 itself. RNA-DNA hybridization-competition studies indicate that the SV40 component of Ad2(+)ND(1) consists of some, but not all, of that part of the SV40 genome which is transcribed early, i.e., prior to viral DNA replication, in SV40 lytic infection.

MeSH terms

  • Adenoviridae / analysis*
  • Animals
  • Antigens, Viral / analysis
  • Base Sequence
  • Carbon Isotopes
  • Cell Line
  • Culture Techniques
  • DNA Replication
  • DNA, Viral / biosynthesis
  • DNA, Viral / isolation & purification
  • Genetic Code
  • Genetics, Microbial
  • Haplorhini
  • Humans
  • Hybridization, Genetic*
  • Kidney
  • Nucleic Acid Hybridization
  • RNA, Viral* / analysis
  • RNA, Viral* / isolation & purification
  • Simian virus 40 / analysis*
  • Simian virus 40 / immunology
  • Simian virus 40 / pathogenicity
  • Thymidine
  • Virus Cultivation

Substances

  • Antigens, Viral
  • Carbon Isotopes
  • DNA, Viral
  • RNA, Viral
  • Thymidine