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    Proc Natl Acad Sci U S A. 1971 Apr;68(4):803-4.

    Identification of 1,25-dihydroxycholecalciferol, a form of vitamin D3 metabolically active in the intestine.

    Holick MF, Schnoes HK, DeLuca HF.

    A biologically active vitamin D(3) metabolite ("peak V metabolite") more polar than 25-hydroxycholecalciferol has been isolated from chicken intestines in pure form as a mono(trimethylsilyl)ether derivative. The molecule has been identified as 1,25-dihydroxycholecalciferol by means of mass spectrometry, ultraviolet absorption spectrophotometry, and specific chemical reactions.

    PMID: 4323790 [PubMed - indexed for MEDLINE]

    PMCID: 389047

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