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    Enzyme. 1979;24(5):294-301.

    Semiautomated enzymic microassay for plasma L-alanine.

    Stahl A, Frick A, Imler M, Schlienger JL.

    Abstract

    Plasma L-alanine is deaminated by bacterial alanine-dehydrogenase; the resulting ammonia is dialyzed out and measured by use of a continuous flow phenol-hypochlorite colorimetric microassay. Concentrations in the range of 10-1,000 mumol alanine/l can be determined in a 50-microliter sample. The optimal conditions for the assay are specified. Study of the analytical qualities of the technique shows high specificity, good reproducibility , and a detection limit of 6 mumol/l. Usual values in human plasma from arterial or venous blood are respectively 296 +/- 166 and 376 +/- 214 mumol alanine/l (x +/- 2 SD). The usual values in rats are close to those found in man.

    PMID:
    41707
    [PubMed - indexed for MEDLINE]

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