The proteolysis of immunoglobulin G with long-acting thyroid-stimulating activity

Biochem J. 1966 Mar;98(3):858-61. doi: 10.1042/bj0980858.

Abstract

1. Proteolysis of immunoglobulin G with long-acting thyroid-stimulating activity with papain and pepsin shortened the duration of thyroid stimulation in mice prepared for the McKenzie (1958) bioassay. The time-course appeared to be related to molecular size. 2. The activity after proteolysis was recovered solely in the electrophoretically slow F(ab)-fragment. 3. There was no immunological cross-reaction between pituitary thyroid-stimulating hormone and the active fragments of the long-acting thyroid stimulator despite their similar behaviour in the bioassay. 4. The implications of these findings are discussed.

MeSH terms

  • Animals
  • Antigen-Antibody Reactions
  • Chemical Phenomena
  • Chemistry
  • Long-Acting Thyroid Stimulator
  • Mice
  • Papain*
  • Pepsin A*
  • Ultracentrifugation
  • gamma-Globulins*

Substances

  • gamma-Globulins
  • Long-Acting Thyroid Stimulator
  • Papain
  • Pepsin A