Abstract
A fraction containing capillaries and rich in gamma-glutamyl transpeptidase was isolated from homogenates of bovine brain cortex by density gradient centrifugation. The enzyme was localized in the endothelial cells by a histochemical procedure. gamma-Glutantyl transpeptidase may function in the transfer of some amino acids across the blood-brain barrier.
MeSH terms
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Alkaline Phosphatase / isolation & purification
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Amino Acids / metabolism*
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Animals
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Blood-Brain Barrier*
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Capillaries / enzymology*
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Cattle
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Centrifugation, Density Gradient
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Cerebral Cortex / blood supply
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Cerebral Cortex / enzymology*
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Endothelium / enzymology
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Nucleotidases / isolation & purification
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gamma-Glutamyltransferase / isolation & purification*
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gamma-Glutamyltransferase / metabolism
Substances
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Amino Acids
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gamma-Glutamyltransferase
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Nucleotidases
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Alkaline Phosphatase