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J Allergy Clin Immunol. 1985 Nov;76(5):724-9.

An immunochemical approach to indoor aeroallergen quantitation with a new volumetric air sampler: studies with mite, roach, cat, mouse, and guinea pig antigens.


We describe a new high-volume air sampler for determining antigen concentrations in homes and illustrate its use for quantitating airborne house dust mite, cat, cockroach, mouse, and guinea pig antigens. The concentration of house dust-mite antigen was similar from houses in Rochester, Minn. and tenement apartments in Harlem, N. Y., but cockroach and mouse urinary proteins were present only in Harlem. The amount of cat or guinea pig antigen varied as expected with the number of pets in the home. In calm air the airborne concentration of mite and cat antigen was similar throughout the house but increased greatly in a bedroom when bedding was changed. In calm air most of the cat and mite antigens were associated with respirable particles less than 5 microns mean aerodynamic mass diameter, but in air sampled after the bedding was changed, more cat antigen was found in particles greater than 5 microns. The apparatus and technique described can provide objective data concerning the magnitude and the relative distribution and duration of suspended particles of defined sizes, which contain allergen activity.

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