J Immunol Methods. 1977;15(3):239-45.

A comparative study of procedures for sheep erythrocyte-human-T-lymphocyte rosette formation.

Mahowald ML, Handwerger BS, Capertone EM Jr, Douglas SD.

Abstract

A comparative study of several published methods for sheep red blood cell-T-lymphocyte rosette formation was performed. Maximum SRBC-rosette formation occurred with AET treated SRBC in medium supplemented with 20% FCS or with untreated SRBC in 100% FCS. Prolongation of the 4 degrees C incubation period from 4 to 18 h enchanced rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette-forming lyphocytes by allowing accurate indentification of the central lymphocytes in morulas.

PMID:: 404361; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Supplemental Content

Save items

Related citations in PubMed

Studies on human T lymphocyte rosette formation with human and sheep erythrocytes. [Res Exp Med (Berl). 1975]
Studies on human T lymphocyte rosette formation with human and sheep erythrocytes.
Cohnen G, Brittinger G. Res Exp Med (Berl). 1975 Nov 26; 166(1):1-13.
A methodological study of E-rosette formation using AET-treated sheep red blood cells. [J Immunol Methods. 1979]
A methodological study of E-rosette formation using AET-treated sheep red blood cells.
Madsen M, Johnsen HE. J Immunol Methods. 1979 May 10; 27(1):61-74.
The E-rosette test: variations in results using E from different sheep. Effect of FCS and of AET treatment. [Acta Pathol Microbiol Immunol ...]
The E-rosette test: variations in results using E from different sheep. Effect of FCS and of AET treatment.
Naess A, Nyland H. Acta Pathol Microbiol Immunol Scand C. 1983 Dec; 91(6):367-70.
Lymphocyte subpopulations. Human red blood cell rosettes. [Clin Exp Immunol. 1975]
Lymphocyte subpopulations. Human red blood cell rosettes.
Tak Yan Yu D. Clin Exp Immunol. 1975 May; 20(2):311-22.
Influence of fetal calf serum on the results of the E rosette test. [Acta Pathol Microbiol Immunol ...]
Influence of fetal calf serum on the results of the E rosette test.
Naess A. Acta Pathol Microbiol Immunol Scand C. 1983 Feb; 91(1):77-9.

See reviews... See all...

Cited by 3 PubMed Central articles

T lymphocyte-derived demyelinating activity in multiple sclerosis patients in relapse. [J Neurol Neurosurg Psychiatry....]
T lymphocyte-derived demyelinating activity in multiple sclerosis patients in relapse.
Selmaj K, Alam R, Perkin GD, Rose FC. J Neurol Neurosurg Psychiatry. 1987 May; 50(5):532-7.
Anti-viral activity induced by culturing lymphocytes with tumor-derived or virus-transformed cells. Enhancement of human natural killer cell activity by interferon and antagonistic inhibition of susceptibility of target cells to lysis. [J Exp Med. 1978]
Anti-viral activity induced by culturing lymphocytes with tumor-derived or virus-transformed cells. Enhancement of human natural killer cell activity by interferon and antagonistic inhibition of susceptibility of target cells to lysis.
Trinchieri G, Santoli D. J Exp Med. 1978 May 1; 147(5):1314-33.
Anti-viral activity induced by culturing lymphocytes with tumor-derived or virus-transformed cells. Identification of the anti-viral activity as interferon and characterization of the human effector lymphocyte subpopulation. [J Exp Med. 1978]
Anti-viral activity induced by culturing lymphocytes with tumor-derived or virus-transformed cells. Identification of the anti-viral activity as interferon and characterization of the human effector lymphocyte subpopulation.
Trinchieri G, Santoli D, Dee RR, Knowles BB. J Exp Med. 1978 May 1; 147(5):1299-1313.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound
PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance
PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

A comparative study of procedures for sheep erythrocyte-human-T-lymphocyte roset...
A comparative study of procedures for sheep erythrocyte-human-T-lymphocyte rosette formation.
J Immunol Methods. 1977 ;15(3):239-45.

PubMed
The influence of body fat distribution on the incidence of diabetes mellitus. 13...
The influence of body fat distribution on the incidence of diabetes mellitus. 13.5 years of follow-up of the participants in the study of men born in 1913.
Diabetes. 1985 Oct ;34(10):1055-8.

PubMed
Neuroma formation and abnormal afferent nerve discharges after partial beak ampu...
Neuroma formation and abnormal afferent nerve discharges after partial beak amputation (beak trimming) in poultry.
Experientia. 1985 Sep 15 ;41(9):1132-4.

PubMed
Uridine diphosphate galactose 4'-epimerase deficiency. IV. Report of eight cases...
Uridine diphosphate galactose 4'-epimerase deficiency. IV. Report of eight cases in three families.
Helv Paediatr Acta. 1977 Apr ;31(6):441-52.

PubMed
The structural gene for human coagulation factor X is located on chromosome 13q3...
The structural gene for human coagulation factor X is located on chromosome 13q34.
Cytogenet Cell Genet. 1985 ;39(3):231-3.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

A comparative study of procedures for sheep erythrocyte-human-T-lymphocyte rosette formation.

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Molecular Biology Databases

Supplemental Content

Save items

Related citations in PubMed

Cited by 3 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information