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Purification and characterisation of leukotriene A4 hydrolase from rat neutrophils.
Leukotriene A4 hydrolase was rapidly and extensively purified from rat neutrophils using anion exchange and gel filtration high-pressure liquid chromatography. The enzyme which converts the allylic epoxide leukotriene A4 to the 5,12-dihydroxyeicosatetraenoic acid leukotriene B4 was localized in the cytosolic fraction and exhibited an optimum activity at pH 7.8 and an apparent Km for leukotriene A4 between 2 X 10(-5) and 3 X 10(-5) M. The purified leukotriene A4 hydrolase was shown to have a molecular weight of 68 000 on sodium dodecylsulfate polyacrylamide gel electrophoresis and of 50 000 by gel filtration. The molecular weight and monomeric native form of this enzyme are unique characteristics which distinguish leukotriene A4 hydrolase from previously purified epoxide hydrolases.
PMID: 3995081 [PubMed - indexed for MEDLINE]
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Cited by 4 PubMed Central articles
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T helper cell type 2 cytokines coordinately regulate immunoglobulin E-dependent cysteinyl leukotriene production by human cord blood-derived mast cells: profound induction of leukotriene C(4) synthase expression by interleukin 4.
Hsieh FH, Lam BK, Penrose JF, Austen KF, Boyce JA.
J Exp Med. 2001 Jan 1; 193(1):123-33.
[J Exp Med. 2001]
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5-Lipoxygenase is located in the euchromatin of the nucleus in resting human alveolar macrophages and translocates to the nuclear envelope upon cell activation.
Woods JW, Coffey MJ, Brock TG, Singer II, Peters-Golden M.
J Clin Invest. 1995 May; 95(5):2035-46.
[J Clin Invest. 1995]
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Molecular cloning and amino acid sequence of leukotriene A4 hydrolase.
Funk CD, Rådmark O, Fu JY, Matsumoto T, Jörnvall H, Shimizu T, Samuelsson B.
Proc Natl Acad Sci U S A. 1987 Oct; 84(19):6677-81.
[Proc Natl Acad Sci U S A. 1987]
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