Anal Biochem. 1985 Apr;146(1):206-10.

A single affinity column step method for the purification of ricin toxin from castor beans (Ricinus communis).

Abstract

A rapid method for purifying ricin toxin from castor beans is presented which uses a single affinity column step to obtain pure toxin from a crude extract of castor beans. A galactosyl-Sepharose affinity matrix was used to bind ricin toxin and its associated agglutinin, which both bind specifically to galactose, from a crude extract. The selective elution of ricin toxin and agglutinin was then achieved by eluting the affinity column with a galactose gradient, which sequentially elutes the two proteins due to a difference in binding avidity to the matrix.

PMID:: 3993931; [PubMed - indexed for MEDLINE]

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Anal Biochem. 1985 Apr ;146(1):206-10.

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