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    Bull World Health Organ. 1979;57 Suppl 1:33-6.

    In vitro propagation of Plasmodium falciparum for merozoite antigens.

    Brackett RG, Cole GC, Green TJ, Jacobs RL.

    Abstract

    A system with continuous flow of medium that uses disposable tissue culture flasks for the in vitro cultivation of P. falciparum is described. This scaled-up modification of the Trager and Jensen method gives merozoite yields sufficient for experimental vaccine studies in animals. A method of increasing the yield of merozoites by decreasing the pH of the medium is presented.

    PMID:
    397007
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC2395713
    Free PMC Article

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