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1: Proc Natl Acad Sci U S A. 1985 Dec;82(24):8429-33.Click here to read Links

Monoclonal antibodies to human vitamin D-binding protein.

Pierce EA, Dame MC, Bouillon R, Van Baelen H, DeLuca HF.

Monoclonal antibodies to vitamin D-binding protein isolated from human serum have been produced. The antibodies obtained have been shown to be specific for human vitamin D-binding protein by three independent assays. The antibodies recognize human vitamin D-binding protein specifically in an enzyme-linked immunosorbent assay. Human vitamin D-binding protein is detected specifically in both pure and crude samples by a radiometric immunosorbent assay (RISA) and by an immunoprecipitation assay. The anti-human vitamin D-binding protein antibodies cross-react with monkey and pig vitamin D-binding protein, but not with vitamin D-binding protein from rat, mouse, or chicken, as determined by the RISA and immunoprecipitation assays.

PMID: 3936035 [PubMed - indexed for MEDLINE]

PMCID: PMC390929