Inhibition of poly(ADP-ribose)polymerase causes increased DNA strand breaks without decreasing strand rejoining in alkylated HeLa cells

FEBS Lett. 1985 Oct 14;190(2):279-82. doi: 10.1016/0014-5793(85)81300-4.

Abstract

Treatment of alkylated HeLa cells with 3-aminobenzamide, an inhibitor of poly(ADP-ribose) polymerase, increased the number of DNA strand breaks but did not affect the rate of strand rejoining. This suggests that an increase in DNA incision, not a decrease in ligation, results from the inhibition of poly(ADP-ribose) polymerase in cells recovering from DNA damaged by alkylating agents.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkylation
  • Benzamides / pharmacology
  • Cytarabine / pharmacology
  • DNA / metabolism*
  • DNA Repair* / drug effects
  • DNA, Neoplasm / metabolism
  • HeLa Cells
  • Humans
  • Hydrogen-Ion Concentration
  • Poly(ADP-ribose) Polymerase Inhibitors*

Substances

  • Benzamides
  • DNA, Neoplasm
  • Poly(ADP-ribose) Polymerase Inhibitors
  • Cytarabine
  • 3-aminobenzamide
  • DNA