Studies of extracellular fibronectin matrix formation with fluoresceinated fibronectin and fibronectin fragments

FEBS Lett. 1985 Apr 22;183(2):365-9. doi: 10.1016/0014-5793(85)80811-5.

Abstract

Fluorescein isothiocyanate conjugated human plasma fibronectin, 70-kDa collagen-binding, 60-kDa central, 60-kDa heparin-binding, 180-kDa heparin, collagen-binding fibronectin fragments and gelatin were used to study extracellular fibronectin matrix formation. Exogenous fibronectin, gelatin, 70-kDa collagen-binding and 180-kDa heparin, collagen-binding fragments were shown to be able to bind specifically to preexisting extracellular matrix of living fibroblasts. The results suggest that: (i) Fibronectin matrix formation may occur through a self-assembly process; (ii) the NH2-terminal part of fibronectin is responsible for fibronectin-fibronectin interaction during fibronectin fibril formation; (iii) plasma fibronectin may be the source for tissue fibronectin.

MeSH terms

  • Cells, Cultured
  • Collagen / metabolism
  • Extracellular Matrix / metabolism*
  • Fibronectins / metabolism*
  • Fluorescein-5-isothiocyanate
  • Fluoresceins / metabolism
  • Fluorescent Antibody Technique
  • Heparin / metabolism
  • Humans
  • Molecular Weight
  • Peptide Fragments / metabolism
  • Skin / cytology
  • Thiocyanates / metabolism

Substances

  • Fibronectins
  • Fluoresceins
  • Peptide Fragments
  • Thiocyanates
  • Heparin
  • Collagen
  • Fluorescein-5-isothiocyanate