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The permeability coefficient for NH3 through bacterial membranes was determined to be around 2 X 10(-3) cm X s-1. This value was used to calculate the outward diffusion of NH3 from intracellular pools and the energy costs for reabsorption as NH4+. For an N2-fixing continuous culture of Klebsiella pneumoniae an energy expenditure of around 4 ATP per NH3 produced was calculated, thus increasing significantly the energy requirement for N2 fixation in vivo.
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