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Nature. 1979 Nov 1;282(5734):39-43.

Isolation and characterisation of a yeast chromosomal replicator.

Abstract

A yeast DNA sequence that behaves as a chromosomal replicator, ars1 (autonomously replicating sequence), has been isolated. On transformation, ars1 allows autonomous replication of all co-linear DNA. The replicator can integrate into other replication units and can function in multimeric form. The 850-base pair ars1 element has no detectable homology to other yeast sequences. Such replicator-containing plasmids can be used for the isolation of DNA sequences in yeast cells as well as for the study of chromosomal DNA replication.

PMID:
388229
[PubMed - indexed for MEDLINE]
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