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Proc Natl Acad Sci U S A. 1985 Nov;82(22):7580-4.

Cloning and expression of the human erythropoietin gene.

Abstract

The human erythropoietin gene has been isolated from a genomic phage library by using mixed 20-mer and 17-mer oligonucleotide probes. The entire coding region of the gene is contained in a 5.4-kilobase HindIII-BamHI fragment. The gene contains four intervening sequences (1562 base pairs) and five exons (582 base pairs). It encodes a 27-amino acid signal peptide and a 166-amino acid mature protein with a calculated Mr of 18,399. The erythropoietin gene, when introduced into Chinese hamster ovary cells, produces erythropoietin that is biologically active in vitro and in vivo.

PMID:
3865178
[PubMed - indexed for MEDLINE]
PMCID:
PMC391376
Free PMC Article
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