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    Proc Natl Acad Sci U S A. 1985 Nov;82(22):7580-4.

    Cloning and expression of the human erythropoietin gene.

    Abstract

    The human erythropoietin gene has been isolated from a genomic phage library by using mixed 20-mer and 17-mer oligonucleotide probes. The entire coding region of the gene is contained in a 5.4-kilobase HindIII-BamHI fragment. The gene contains four intervening sequences (1562 base pairs) and five exons (582 base pairs). It encodes a 27-amino acid signal peptide and a 166-amino acid mature protein with a calculated Mr of 18,399. The erythropoietin gene, when introduced into Chinese hamster ovary cells, produces erythropoietin that is biologically active in vitro and in vivo.

    PMID:
    3865178
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC391376
    Free PMC Article

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