Brain Sample Preparation After Performing in Utero Electroporation to Proliferating and Differentiated Cells in the Developing Mouse Neocortex

Minkyung Shin; Yoichi Kosodo

doi:10.1007/978-1-0716-3810-1_16

Brain Sample Preparation After Performing in Utero Electroporation to Proliferating and Differentiated Cells in the Developing Mouse Neocortex

Methods Mol Biol. 2024:2794:187-200. doi: 10.1007/978-1-0716-3810-1_16.

Authors

Minkyung Shin¹, Yoichi Kosodo^{2

3}

Affiliations

¹ Korea Brain Research Institute (KBRI), Daegu, Republic of Korea.
² Korea Brain Research Institute (KBRI), Daegu, Republic of Korea. kosodo@kbri.re.kr.
³ Department of Brain Sciences, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, Republic of Korea. kosodo@kbri.re.kr.

PMID: 38630230
DOI: 10.1007/978-1-0716-3810-1_16

Abstract

In utero electroporation (IUE) enables labeling and manipulating specific types of cells by introducing DNA plasmids with desired promoters. After the surgery, mouse brains are fixed at any stage and analyzed after staining using specific antibodies. Here, we describe the flow of the IUE experiment from the preparation to microscopic observations.

Keywords: Brain dissection; Cryostat; DNA plasmid; Electroporator; Immunohistochemistry; In utero electroporation; Microcapillary.

MeSH terms

Animals
Antibodies
Cell Differentiation
Electroporation*
Mice
Neocortex*
Specimen Handling

Substances

Antibodies