Bioaccumulation efficacy and physio-morphological adaptations in response to iron and aluminium contamination of Indian camphorweed (Pluchea indica L.) using different growth substrates

The aim of this study was to assess the removal capability of Fe/Al contamination of Indian camphorweed (Pluchea indica; hereafter, P. indica) using different growth substrates (100% sand, gardening soil, vermiculite, and zeolite). In addition, the study aimed at observing the physio-morphological adaptation strategies of P. indica under excess Fe/Al levels in a controlled greenhouse environment. After a 4-week treatment, P. indica plants under excess Fe in the 100% sand substrate exhibited signs of decay and eventually death. In contrast, the growth performances of P. indica under gardening soil substrate remained sustained even when exposed to Fe/Al stress. Under zeolite substrate, Fe in the root tissues was 23.1 and 34.7 mg g^-1 DW after 1 and 4 weeks of incubation, respectively. In addition, Al in the root tissues also increased to 1.54 mg g^-1 DW after 1 week and 1.59 mg g^-1 DW after 4 weeks, when subjected to 20 mM Al treatment. Zeolite was observed to be a promising substrate to regulate the uptake of Fe (3.31 mg plant^-1) and Al (0.51 mg plant^-1) by the root tissues. The restriction of Fe and Al in the root and a low translocation to the leaf organ was indicated by a low translocation factor (< 1.0). High Fe concentrations in the root and leaf tissues negatively affected root elongation, and the net photosynthetic rate decreased by > 40% compared to positive control. Gas exchange parameters and leaf temperature were found the most sensitive to Fe/Al stress. Moreover, the limited transpiration rate under Fe/Al stress caused an increase of the leaf temperature and crop stress index. The findings suggest that P. indica grown using zeolite substrate may serve as a good model system for constructed wetlands, storing excess Al in the root tissues without any significant growth inhibition.