Fabrication of peptide-encapsulated sodium alginate hydrogel for selective gallium adsorption

Peptides are recognized as promising adsorbents in metal selective recovery. In this study, the designed gallium-binding peptide H₆GaBP was immobilized by the polysaccharide polymer sodium alginate (SA) for gallium recovery. The synthesized H₆GaBP@SA microspheres exhibited a maximum adsorption capacity of 127.4 mg/g and demonstrated high selectivity for gallium at lower pH values. The adsorption process aligned well with the pseudo-second-order equation and Langmuir model. To elucidate the adsorption mechanism, a comprehensive characterization including molecular docking, scanning electron microscope coupled with energy-dispersive X-ray spectroscopy (SEM-EDS), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and thermogravimetry analysis (TGA), were conducted. These analyses revealed that gallium ions were initially adsorbed through electrostatic interaction by H₆GaBP@SA, followed by a cation exchange reaction between Ga(OH)²⁺ and Ca²⁺, as well as coordination between gallium and histidine residues on the peptide. Moreover, the H₆GaBP@SA exhibited improved thermal stability compared to sole sodium alginate microspheres, and the coordination of gallium with peptides can also defer the decomposition rate of the adsorbents. Compared to other adsorbents, the peptide-encapsulated hydrogel microspheres exhibited superior gallium selectivity and improved adsorption capacity, offering an environmentally friendly option for gallium recovery.