-
Molecular cloning of complementary DNA for the alpha subunit of the G protein that stimulates adenylate cyclase.
A complementary DNA clone encoding the alpha subunit of the adenylate cyclase stimulatory G protein (Gs) was isolated and identified. A bovine brain complementary DNA library was screened with an oligonucleotide probe derived from amino acid sequence common to known G proteins. The only clone that was obtained with this probe has a complementary DNA insert of approximately 1670 base pairs. An antibody to a peptide synthesized according to deduced amino acid sequence reacts specifically with the alpha subunit of Gs. In addition, RNA that hybridizes with probes made from the clone is detected in wild-type S49 cells; however, cyc- S49 cells, which are deficient in Gs alpha activity, are devoid of this messenger RNA.
PMID: 3839937 [PubMed - indexed for MEDLINE]
-
Cited by 34 PubMed Central articles
-
Genetic analysis of the Drosophila Gs(alpha) gene.
Wolfgang WJ, Hoskote A, Roberts IJ, Jackson S, Forte M.
Genetics. 2001 Jul; 158(3):1189-201.
[Genetics. 2001]
-
Phospholipase C-delta1 and oxytocin receptor signalling: evidence of its role as an effector.
Park ES, Won JH, Han KJ, Suh PG, Ryu SH, Lee HS, Yun HY, Kwon NS, Baek KJ.
Biochem J. 1998 Apr 1; 331 ( Pt 1):283-9.
[Biochem J. 1998]
-
Oxytocin receptor couples to the 80 kDa Gh alpha family protein in human myometrium.
Baek KJ, Kwon NS, Lee HS, Kim MS, Muralidhar P, Im MJ.
Biochem J. 1996 May 1; 315 ( Pt 3):739-44.
[Biochem J. 1996]
- » See all...