Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole

Weikang Zhang; Yingwu Lin; Xiangmin Meng; Qiaoning Wang; Lingxin Chen; Jiakun Xu

doi:10.1016/j.jhazmat.2023.133147

Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole

J Hazard Mater. 2024 Mar 5:465:133147. doi: 10.1016/j.jhazmat.2023.133147. Epub 2023 Dec 1.

Authors

Weikang Zhang¹, Yingwu Lin², Xiangmin Meng³, Qiaoning Wang⁴, Lingxin Chen⁵, Jiakun Xu⁶

Affiliations

¹ State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Lab for Marine Drugs and Byproducts of Pilot National Lab for Marine Science and Technology, Qingdao 266071, China; College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
² School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China.
³ College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
⁴ CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
⁵ CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China. Electronic address: lxchen@yic.ac.cn.
⁶ State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Lab for Marine Drugs and Byproducts of Pilot National Lab for Marine Science and Technology, Qingdao 266071, China. Electronic address: xujk@ysfri.ac.cn.

PMID: 38056266
DOI: 10.1016/j.jhazmat.2023.133147

Abstract

Sulfonamide antibiotics, a family of broad-spectrum antibiotic drugs, are increasingly used in aquaculture and are frequently detected in aquatic environments. This poses a potential threat to organisms and may cause the evolution of antimicrobial resistance. Therefore, it is important to develop an environmentally friendly and efficient biocatalyst to degrade sulfonamides (SAs) such as sulfadiazine (SD) and sulfathiazole (ST). Here, we realized the direct and efficient degradation of SD and ST using a hydrogen peroxide-dependent artificial catalytic system based on myoglobin (Mb). The arrangements of amino acids at positions 29, 43, 64, and 68 were found to influence catalytic activity. An L29H/H64D/V68I myoglobin mutant showed the best catalytic efficiency (i.e., k_cat/K_m = 720.42 M^-1 s^-1) against SD. Next, mutant H64D/V68I showed the best degradation rate against SD (i.e., 91.45 ± 0.16%). Moreover, L29H/H64D/V68I Mb was found to efficiently catalyze ST oxidation (k_cat/K_m = 670.08 M^-1 s^-1), while H64D/V68I had the best degradation rate against ST (i.e., 99.45 ± 0.23%). Our results demonstrate that SAs can be efficiently degraded by artificial peroxygenases constructed using a myoglobin scaffold. This therefore provides a simple and economical method for the biodegradation of SD and ST.

Keywords: Biocatalysis; Biodegradation; Directed evolution; Myoglobin; Sulfonamides.

MeSH terms

Amino Acids / metabolism
Anti-Bacterial Agents
Myoglobin* / chemistry
Myoglobin* / metabolism
Sulfadiazine*
Sulfathiazole
Sulfonamides

Substances

Myoglobin
Sulfadiazine
Anti-Bacterial Agents
Amino Acids
Sulfathiazole
Sulfonamides