A micro-nano interface integrated SERS-based microfluidic sensor for miRNA detection using DNAzyme walker amplification

Yang Lu; Yiyue Yu; Yeru Wang; Wenbo Zhou; Ziyi Cheng; Liandong Yu; Shaojiang Zheng; Rongke Gao

doi:10.1016/j.aca.2023.341957

A micro-nano interface integrated SERS-based microfluidic sensor for miRNA detection using DNAzyme walker amplification

Anal Chim Acta. 2023 Dec 1:1283:341957. doi: 10.1016/j.aca.2023.341957. Epub 2023 Oct 24.

Authors

Yang Lu¹, Yiyue Yu¹, Yeru Wang¹, Wenbo Zhou¹, Ziyi Cheng², Liandong Yu³, Shaojiang Zheng⁴, Rongke Gao⁵

Affiliations

¹ College of Control Science and Engineering, China University of Petroleum (East China), Qingdao, 266580, China.
² Hainan Cancer Medical Center of The First Affiliated Hospital, Key Laboratory of Tropical Cardiovascular Diseases Research of Hainan Province, Key Laboratory of Emergency and Trauma of Ministry of Education, Hainan Women and Children Medical Center, Hainan Medical University, Haikou, 571199, China.
³ College of Control Science and Engineering, China University of Petroleum (East China), Qingdao, 266580, China. Electronic address: liandongyu@upc.edu.cn.
⁴ Hainan Cancer Medical Center of The First Affiliated Hospital, Key Laboratory of Tropical Cardiovascular Diseases Research of Hainan Province, Key Laboratory of Emergency and Trauma of Ministry of Education, Hainan Women and Children Medical Center, Hainan Medical University, Haikou, 571199, China. Electronic address: zhengshaojiang@hainmc.edu.cn.
⁵ College of Control Science and Engineering, China University of Petroleum (East China), Qingdao, 266580, China. Electronic address: rkgao@upc.edu.cn.

PMID: 37977782
DOI: 10.1016/j.aca.2023.341957

Abstract

Background: Precise and specific miRNA detection plays a vital role in exploring development mechanisms of cancer disease, thereby it can significantly improve relevant prevention and treatment strategies.

Results: In this work, a surface-enhanced Raman spectroscopy (SERS)-based microfluidic chip has been devised with a microcone array SERS substrate (MCASS) for the miR-141 detection. This substrate excels in unique SERS activity and large surface area for DNA oligonucleotide modification. As the presence of miR-141, the DNAzyme walker induced cleavage reaction took place on the finely designed and prepared dual DNA conjugated SERS nanoprobes. The SERS nanoprobes can anchor on MCASS by the DNA hybridization that achieved an impressive detection limit in the femtomolar level.

Significance: With this integrated SERS-based microfluidic chip, we provided a miRNA detection strategy using DNAzyme walker amplification technology. It is believed that this strategy could be a powerful tool for miRNA detection and related cancer screening test.

Keywords: DNAzyme walker; Micro-nano interface; Microfluidics; SERS; miRNAs.

MeSH terms

DNA
DNA, Catalytic*
Limit of Detection
Metal Nanoparticles* / chemistry
MicroRNAs* / chemistry
Microfluidics
Spectrum Analysis, Raman / methods

Substances

MicroRNAs
DNA, Catalytic
DNA