Comparative evaluation of in-house developed latex agglutination test (LAT) with World Organisation for Animal Health (WOAH) -recommended methods for the detection of Bacillus anthracis spores from the soil

J Microbiol Methods. 2023 Aug:211:106778. doi: 10.1016/j.mimet.2023.106778. Epub 2023 Jun 30.

Abstract

In-house developed Bacillus anthracis-specific synthetic peptide-based latex agglutination test (LAT) assay was comparatively evaluated with World Organisation for Animal Health (WOAH)-recommended polymerase chain reaction (PCR)/real-time PCR (qPCR) methods for the screening of B. anthracis spores from the soil to provide a simple, rapid, and economical immunodiagnostic test for field application.

Keywords: Bacillus anthracis; Latex agglutination test; Real-time PCR; Soil; Spores; Synthetic peptides.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus anthracis* / isolation & purification
  • Bacteriological Techniques* / methods
  • Bacteriological Techniques* / standards
  • Latex Fixation Tests* / standards
  • Limit of Detection
  • Real-Time Polymerase Chain Reaction / standards
  • Soil Microbiology
  • Spores, Bacterial* / isolation & purification