Genome-Wide Identification of NAC Gene Family and Expression Analysis under Abiotic Stresses in Avena sativa

Lei Ling; Mingjing Li; Naiyu Chen; Xinying Xie; Zihui Han; Guoling Ren; Yajie Yin; Huixin Jiang

doi:10.3390/genes14061186

Genome-Wide Identification of NAC Gene Family and Expression Analysis under Abiotic Stresses in Avena sativa

Genes (Basel). 2023 May 29;14(6):1186. doi: 10.3390/genes14061186.

Authors

Lei Ling¹, Mingjing Li¹, Naiyu Chen¹, Xinying Xie¹, Zihui Han¹, Guoling Ren¹, Yajie Yin¹, Huixin Jiang¹

Affiliation

¹ Heilongjiang Provincial Key Laboratory of Oilfield Applied Chemistry and Technology, College of Bioengineering, Daqing Normal University, Daqing 163712, China.

Abstract

In this study, a total of 177 NAC members were identified in Avena sativa, located on 21 chromosomes. Phylogenetic analysis showed that AsNAC proteins could be divided into seven subfamilies (I-VII), and that proteins in the same subfamily have similar protein motifs. Gene structure analysis found that NAC introns ranged from 1 to 17. Cis-element analysis of the promoter indicated that the gene family may have stress-related elements and growth regulation elements. Through qRT-PCR experiments, we speculated that AsNACs genes can respond to abiotic stresses such as cold, freezing, salt, and saline alkali. This study provides a theoretical basis for further exploring the function of the NAC gene family in A. sativa.

Keywords: Avena sativa; NAC transcription factor; evolutionary analysis: gene expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Avena* / genetics
Introns / genetics
Phylogeny
Promoter Regions, Genetic
Stress, Physiological* / genetics

Grants and funding

This work was supported by a grant from the Daqing Normal University Doctoral Research Fund China (No. 21ZR05) and the Heilongjiang Natural Science Foundation Joint Guiding Project (LH2022C001).