Gene. 1987;56(2-3):253-65.

Splicing of plant pre-mRNAs in animal systems and vice versa.

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Laboratory of Genetics, University of Wisconsin, Madison 53706.

Abstract

To investigate similarities and differences of pre-mRNA splicing between higher plants and animals, we tested whether pre-mRNAs of higher plants can be accurately spliced in animal systems, and whether an animal pre-mRNA can be spliced in plant cells. Pre-mRNAs of the maize bronze locus and oat phytochrome type 3 are accurately spliced with moderate efficiency in a human (HeLa cell) nuclear extract. The first intervening sequence (IVS1) of bean phaseolin pre-mRNA is not excised in the human nuclear extract, but is removed (although at low efficiency) in intact monkey cells. However, the IVS1 of human alpha-globin pre-mRNA is not removed in tobacco cells. Our data suggest that the mechanisms of pre-mRNA splicing are similar, but not identical, in plants and animals.

PMID:: 3678838; [PubMed - indexed for MEDLINE]

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Research Support, U.S. Gov't, Non-P.H.S.

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