Genes Dev. 1987 Aug;1(6):585-93.

Isolation and characterization of the active cDNA of the human cell cycle gene (RCC1) involved in the regulation of onset of chromosome condensation.

Ohtsubo M, Kai R, Furuno N, Sekiguchi T, Sekiguchi M, Hayashida H, Kuma K, Miyata T, Fukushige S, Murotsu T, et al.

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Laboratory of Molecular Genetics, Faculty of Science, Kyushu University, Fukuoka, Japan.

Abstract

The human RCC1 gene was cloned after DNA-mediated gene transfer into the tsBN2 cell line, which shows premature chromosome condensation at nonpermissive temperatures (39.5-40 degrees C). This gene codes for a 2.5-kb poly(A)+ RNA that is well conserved in hamsters and humans. We isolated 15 cDNA clones from the Okayama-Berg human cDNA library, and found two that can complement the tsBN2 mutation with an efficiency comparable to that of the genomic DNA clone. The base sequences of these two active cDNA clones differ at the 5' proximal end, yet both have a common open reading frame, encoding a protein of 421 amino acids with a calculated molecular weight of 44,847 and with seven homologous repeated domains of about 60 amino acids. This human RCC1 gene was located to human chromosome 1 using sorted chromosomal fractions.

PMID:: 3678831; [PubMed - indexed for MEDLINE]

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anti-Regulator of Chromosome Condensation 1 (RCC1) (AA 19-176) antibody - antibodies-online

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Cloning of Xenopus RCC1 cDNA, a homolog of the human RCC1 gene: complementation of tsBN2 mutation and identification of the product. [J Biochem. 1990]
Cloning of Xenopus RCC1 cDNA, a homolog of the human RCC1 gene: complementation of tsBN2 mutation and identification of the product.
Nishitani H, Kobayashi H, Ohtsubo M, Nishimoto T. J Biochem. 1990 Feb; 107(2):228-35.
Premature chromosome condensation is induced by a point mutation in the hamster RCC1 gene. [Mol Cell Biol. 1990]
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Ittmann M, Greco A, Basilico C. Mol Cell Biol. 1987 Oct; 7(10):3386-93.
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Review The 'BN2' gene, a regulator for the onset of chromosome condensation.
Nishimoto T. Bioessays. 1988 Oct; 9(4):121-4.
Review The discovery (or rediscovery?) of the phenomenon of premature chromosome condensation. [Bioessays. 1990]
Review The discovery (or rediscovery?) of the phenomenon of premature chromosome condensation.
Rao PN. Bioessays. 1990 Apr; 12(4):193-7.

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Isolation and characterization of the active cDNA of the human cell cycle gene (RCC1) involved in the regulation of onset of chromosome condensation.
Genes Dev. 1987 Aug ;1(6):585-93.

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