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    Biochem Biophys Res Commun. 1987 Oct 14;148(1):232-8.

    Purification and characterization of human liver cytochrome P-450-ALC.

    Lasker JM, Raucy J, Kubota S, Bloswick BP, Black M, Lieber CS.

    Source

    Alcohol Research and Treatment Center, Bronx Veterans Administration Medical Center, NY.

    Abstract

    Cytochrome P-450-ALC, an ethanol-oxidizing form of microsomal cytochrome P-450 (P-450), has been purified from human liver. P-450-ALC (Mr = 54,000 daltons) is a low-spin ferric hemeprotein with a CO-reduced Soret maximum at 452 nm, and has an NH2-terminal amino acid sequence nearly identical to that deduced from a human P-450-ALC cDNA clone. In a reconstituted system, P-450-ALC oxidizes ethanol and aniline at turnover rates (12.2 and 7.3 nmol min-1, respectively) 10-fold greater than two other human P-450 isozymes (termed P-450-B and P-450-C) purified from the same liver. Both P-450-ALC and P-450-C effectively demethylate N-nitrosodimethylamine (NDMA) at low substrate concentrations (0.5 mM), especially in the presence of cytochrome b5. Our results provide direct evidence for a liver P-450 isozyme in humans with catalytic properties similar to the related alcohol-inducible rodent P-450s and also reveal a new human NDMA demethylase.

    PMID:
    3675576
    [PubMed - indexed for MEDLINE]

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